database search using peaks studio 5.2 Search Results


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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Validation of the <t>proteome</t> data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image <t>J</t> <t>software</t> and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.
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Image Search Results


Validation of the proteome data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image J software and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Integrated Transcriptomic and Proteomic Analysis of the Global Response of Synechococcus to High Light Stress *

doi: 10.1074/mcp.M114.046003

Figure Lengend Snippet: Validation of the proteome data and transcriptome data. A, Comparison of log2 expression of 20 selected differentially regulated genes measured by RNA-Seq and qRT-PCR. Positive and negative log2 expression ratios represent up- and down-regulation after HL exposure, respectively. Each data point is calculated from averages of biological triplicates. B, Results derived from RNA-Seq analysis (white bars; n = 2) were compared with those from qRT-PCR (gray bars; n = 3). C, Western blot analysis of protein expression levels of five representative proteins quantified by proteomics after HL exposure. Fold changes of protein levels were determined using Image J software and normalized by protein concentration. D, The mRNA expression level of the validated proteins as detected by qRT-PCR.

Article Snippet: Database Search Raw data files were processed to generate peak list files using Proteome Discoverer software (Thermo Fisher Scientific, v. 1.3.0.339).

Techniques: Expressing, RNA Sequencing Assay, Quantitative RT-PCR, Derivative Assay, Western Blot, Software, Protein Concentration